{"id":1599,"date":"2020-03-26T14:39:56","date_gmt":"2020-03-26T07:39:56","guid":{"rendered":"http:\/\/www.neuroscienceres.com\/?p=1599"},"modified":"2022-01-07T10:09:18","modified_gmt":"2022-01-07T03:09:18","slug":"addition-mz-cells-enhanced-secretory-apparatus-activation","status":"publish","type":"post","link":"http:\/\/www.neuroscienceres.com\/index.php\/2020\/03\/26\/addition-mz-cells-enhanced-secretory-apparatus-activation\/","title":{"rendered":"In addition MZ B cells have an enhanced secretory apparatus and lower activation"},"content":{"rendered":"

Therefore, CpG ODN and LPS are not synergy in B cell proliferation due to the synergistic induction of large amounts of IL-10. Consistent with these findings, we observed that bacterial genomic DNA also greatly enhanced LPStriggered IL-10 production, but to a lesser extent than CpG ODN. Bacterial DNA and LPS had neither additive nor synergetic effect on na\u0131 \u00a8ve B cell proliferation, although both stimulants were potent mitogens for B cells. In addition, the immunosuppressive effect of IL-10 on CpG ODN-stimulated Ig-secreting cell generation was also previously reported. Similarly, we found that stimulation with bacterial DNA along with LPS did not enhance differentiation into plasma cells. In contrast, stimulation with ALD-DNA alone had no effect on IL-10 expression and slightly increased Doxorubicin<\/a> LPS-triggered IL-10 production. Taken together, the data indicate that ALD-DNA is functionally distinct from CpG ODN and bacterial genomic DNA, and is able to enhance LPS-mediated proliferation and plasma cell generation in part due to its weak ability to enhance LPS-triggered IL-10 production. Remarkably, we found that ALD-DNA treated lupus B cells were able to induce surface CD138 and secrete IgM. ALD-DNA slightly increased XBP1 and Blimp-1 mRNA expression. These data suggested that ALD-DNA at least partially activated plasma cell differentiation and promoted a subset of lupus B cells to become IgM-secreting plasmablasts\/plasma cells. In contrast, ALD-DNA alone had little effect on the increases in CD138 cell numbers, antibody production and mRNA levels of XBP1 and Blimp-1 in normal naive B cells. Therefore, lupus B cells appear to be more sensitive to ALD-DNA stimulation than normal B cells. Furthermore, ALD-DNA enhanced LPS-triggered plasmablast\/plasma cell differentiation program of lupus B cells, as evidenced by increases in CD138 + cell numbers, XBP1 and Blimp-1 mRNA expression as well as IgM production. The ELISA data indicated that ALD-DNA enhanced LPSinduced IgG, but not IgM, production in normal na\u0131 \u00a8ve B cells. In striking contrast, ALD-DNA promoted LPS-induced IgM, but not IgG, production in lupus B cells. Therefore, ALD-DNA had distinct effect on antibody production in normal and lupus B cells, at least under these in vitro culture conditions. IL-6 is a survival factor for plasma cells and acts as a nonswitching factor to enhance IgG production by committed B cells. ALD-DNA promoted LPS-induced IL-6 expression in normal na\u0131 \u00a8ve B cells and this finding may in part account for the increased IgG production. Conversely, ALD-DNA did not enhance LPS-induced IL-6 expression in lupus B cells. The mechanisms that enable ALD-DNA to selectively stimulate IgM production by lupus B cells, but not normal B cells, remain unclear. Moreover, lupus B cells showed higher responsiveness than normal B cells to ALD-DNA and\/or LPS stimulation to undergo terminal differentiation and yielded higher antibody production. These differential effects might be partially attributable to the distinct B cell subset composition. Our result also confirmed this abnormality. MZ B cells are programmed for efficient differentiation into mature plasma cells with the ability to secrete massive quantities of IgM in response to TLR agonists such as LPS.<\/p>\n","protected":false},"excerpt":{"rendered":"

Therefore, CpG ODN and LPS are not synergy in B cell proliferation due to the synergistic induction of large amounts of IL-10. Consistent with these findings, we observed that bacterial genomic DNA also greatly enhanced LPStriggered IL-10 production, but to a lesser extent than CpG ODN. Bacterial DNA and LPS had neither additive nor synergetic … Continue reading In addition MZ B cells have an enhanced secretory apparatus and lower activation<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/posts\/1599"}],"collection":[{"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/comments?post=1599"}],"version-history":[{"count":1,"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/posts\/1599\/revisions"}],"predecessor-version":[{"id":1600,"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/posts\/1599\/revisions\/1600"}],"wp:attachment":[{"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/media?parent=1599"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/categories?post=1599"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.neuroscienceres.com\/index.php\/wp-json\/wp\/v2\/tags?post=1599"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}