Several bilayer interaction and disruption models have been proposed for those AMPs that depend on membrane interference for their antibacterial activity, such as “barrel-stave pore”, “carpet mechanism”, “toroidal pore” and “disordered toroidal pore”. New molecular models has been proposed to describe the mechanism more accurately, such as “form ion channels” and “internalization into the cytoplasm”. In the present study, the results of secondary structure Dabrafenib analysis, bactericidal assays, enzyme release assays provided solid evidence that the inhibitory effect of Kn2-7 on the bacteria was mediated through rapid killing. However, the binding mode of Kn2-7 to the surface of E. coli seems different from that of S. aureus. The results of biolayer interferometry and competition binding assay proved to be so. The findings of TEM indicated that the bacterial cell wall of S. aureus was disrupted immediately upon treatment with Kn2-7. Kn2-7 accumulated on the surface of E. coli, combined with the outer membrane. This mode is consistent with the model of carpet mechanism in general. To the best of our knowledge, the structures of the microspheres were first observed in the antibacterial mechanism study and our results provide conclusive evidence for the model of carpet mechanism. AMPs have both a cationic and amphiphilic nature. Their positive net charge ensures that AMPs can accumulate at the surface of bacteria that contain anionic polymers, such as LTA and LPS. Then, these peptides interact with the membranes of the microbes, resulting in membrane disruption. The results of BLI titrations and competition binding assays showed that Kn2-7 disrupted the cell walls of S. aureus and E. coli through binding to LTA and LPS, respectively. The question of why the mutant peptide Kn2-7 can bind to both LTA and LPS while the wild-type peptide BmKn2 can bind to only LTA is of particular interest. Kn2-7 has five net positive charges while BmKn2 only has two. Therefore we speculate that increasing the numbers of positively charged residues turned out to have improved the affinity to the bacterial surface. Primary liver cancer is the sixth most common cancer worldwide. Due to its poor prognosis and high fatality rates, the incidence and mortality rates are almost equal. Primary liver cancer causes 695,900 deaths every year, making it the third most common cause of cancer-related deaths. Hepatocellular carcinoma, which represents the dominant histological type, accounts for 70–85% of primary malignancies in liver. Epidemiological survey suggests that East and South-East Asia and Middle andWestern Africa havethe highest prevalence of HCC with almosthalf ofthenewcasesand deathsinChina.Althoughchronic infection of hepatitisBvirusand hepatitisCvirusare consideredasthe major riskfactors ofHCC,theetiology ofHCCis stillyettobeclarified. Current studies indicated genetic factors may also contribute to the etiology of HCC. MicroRNAs are small non-coding, single-stranded RNA molecules with typical length of,22 nucleotides.