In our study, Zta was sufficient to induce SOCS3 expression and inhibit STAT2 phosphorylation upon IFNa stimulation of monocytes. Whilst SOCS3 expression could not be recapitulated with mutated Zta, STAT2 phosphorylation was only partly restored following IFNa treatment in this context. The DZta vector encodes the full-lenght Zta protein with two amino acid substitutions in the transactivation domain, only affecting part of its transcriptional activity. Thus, DZta-mediated activation of other IFN signaling modulatory factors may account for the incomplete restoration of STAT2 phosphorylation. A possible factor is IL-10, which is known to be activated by Zta and to inhibit IFNa-induced phosphorylation of STAT proteins. Certainly, the pleiotropic action of Zta during EBV infection is only beginning to be fully revealed and its dual effects may be clarified through future investigations. As AII amacrine cells are the most abundant glycinergic subtype in the rat retina, we investigated whether Sez-6 was expressed in this subtype however we observed no colocalization of Sez-6 and parvalbumin in AII amacrine cells. Nevertheless, Sez-6 may serve as a useful marker of amacrine cells in the rodent retina as it is expressed by the majority of GABAergic amacrine subtypes and some glycinergic amacrine cells. Additionally, the Sez-6 antibody weakly labelled other neuronal cell types including bipolar and horizontal cells although specific Sez-6 immunostaining was notably absent from photoreceptors. The interaction of aSyn with synaptic vesicles is highly dynamic, which may also explain the variety of aSyn conformations detected throughout the axons and dendrites. To confirm staging of late diestrus samples and milk production, we co-stained our tissues with wheat germ agglutin, which stains the apical membrane of actively lactating mammary glands. Results showed that PAD2 expression also strongly correlates with WGA staining at diestrus, thus potentially linking PAD2 expression with lactation. Finally, we used TUNEL staining to determine if any of our estrous cycle staged mammary tissue samples show high levels of apoptosis as occurs during mammary gland involution. None of our samples showed a high degree of apoptosis/necrosis indicating that the tissues were not actively involuting; however the anestrus sections did display the most apoptotic cells. Thus, by both morphological and cellular IF labeling, PAD2 expression varies over the course of the canine estrous cycle with highest expression levels Oligomycin A appearing in alveolar unit epithelium during diestrus. In those experiments, PyMT induced cystic papillary neoplasms with ductal differentiation and pancreatic intraepithelial neoplasia lesions, as well as carcinomas with mixed acinar and endocrine components, whereas cMyc induced endocrine neoplasms that express insulin.