Which can be robust to environmental perturbations as in the cases of transporters and receptors, or be flexible to the immediate microenvironments such as the cases of glycoenzymes and extracellular proteins. Using ordinary LC-MS, we demonstrated here the power of a high-throughput proteomics in studies of post-translational modification from a systematic viewpoint, and we hope that the obtained N-glycoproteome of E14.Tg2a will help a better understanding of the molecular background of this important cell line. For the last couple of decades, researchers have experimented several types and methods of extraction to observe a wide array of pharmacological properties, including limitation of the epithelial cell death in response to oxidant stress, amelioration of the oedema via inhibition of cyclooxygenase-1 and 22, cytoprotection by means of free radical scavenging, reduction of oxidative stress and direct inhibition of the TNF-a production, to name a few. Most of these researchers have attributed the biological effects of U. tomentosa to the pentacyclic oxindole Folinic acid calcium salt pentahydrate alkaloids present in this plant. This idea is supported by the apparently restricted occurrence of these alkaloids within this genus, as well as by studies performed with alkaloids isolated from the plant rather than with brute extracts. Indeed, reference to the use of alkaloids isolated from U. tomentosa goes as far back as to 1985, when Wagner and colleagues observed that four out of six oxindole alkaloids present in this plant caused a pronounced enhancement of phagocytosis, both in vitro and in vivo. More recently, studies with several of these isolated alkaloids yielded reports on their antioxidant, immunomodulant and even anti-neoplastic properties. Other studies, however, have demonstrated that compounds different than the oxindole alkaloids may be at least partially responsible for the 
observed effects. Among the most cited such substances are triterpenes, quinovic acid glycosides, and others hitherto not identified. Yet a third hypothesis, albeit older, suggests that the LOUREIRIN-B anti-inflammatory properties of UT may be related to a synergic combination of compounds. Currently, growing attention is being paid to the anti-neoplastic potential of U. tomentosa. Indeed, various extracts and compounds derived from this plant have been found to alter or downright inhibit the growth and proliferation of several different tumour lineages including human neuroblastoma and glioma, HL60 promyelocytic leukaemia and MCF7 breast cancer, among others. Also, our team of researchers published one of the first studies of the anti-neoplastic properties of U. tomentosa on a solid tumour in vivo. In addition to observing an important reduction of the tumour mass and volume as a result of the treatment with a hydroethanolic extract of the plant, we noted a remarkable modulation of the oxidative stress caused by the neoplastic process, both in the liver and in the tumour of the subjects. Our conclusion was that the modulation of the redox processes would probably play a pivotal role in the antiproliferative effects of the plant, perhaps via alteration of one or more metabolic pathways. It is only logical to assume that all these anti-neoplastic properties should be due to the pentacyclic oxindole alkaloids, which have been shown to exert the anti-inflammatory properties mentioned above.