Direct thermal insult usually results in irreversible tissue damage within the zone of stasis of burn wounds; however, the extent and area of thermal damage are not restricted to the initial region of injury. Therefore, the periburn zones, including those of stasis and hyperaemia, are at risk of wound progression, which may deepen and extend the existing wound. The skin tissue exhibits early responses to heat stimuli, including direct heat-induced protein denaturation and cell death. Complex factors contribute to further development of the burn wound, such as tissue hypoperfusion, injury through oxygen free radicals and inflammation, while autophagy may play a vital role in wound progression via possible Moxifloxacin hydrochloride mechanisms of survival or nonapoptotic programmed cell death. Moreover, the conversion of burn wounds to full-thickness will result in more severe consequences, such as slow Vinorelbine healing wounds, systemic inflammation, local infection and even sepsis, which lead to a poor prognosis and high risk of mortality. Previous studies have focused on the process of wound progression and effective routines and proper time windows for intervention. Considering its reported effects on oxidative stress, apoptosis and inflammation in multiple disease models, hydrogen maybe of value during wound progression. Here, we investigated for the first time the possible preventative effects of hydrogen on transforming the zone of stasis. The results indicate that HS administration significantly attenuates the oxidative stress during early stages in burn wounds by inhibiting membrane lipid peroxidation and enhancing the activity of endogenous antioxidant enzyme systems. In addition, HS treatment markedly inhibited apoptosis and autophagy in the interspace wound tissues after burns. Furthermore, HS application also remarkably decreased protein expression of pro-inflammatory cytokines, and upregulated protein expression of the anti inflammatory cytokine IL-10.The expression of signal proteins, Akt and NF-��B, was significantly repressed after HS management.