Of the down-regulated proteins, Gammaactin is an ubiquitous protein involved in the formation of filaments that are a major component of the cytoskeleton. Abnormal CHEmotaxis family member is one of the AAA+ superfamily. In Caenorhabditis elegans Che-3 is specifically responsible for the retrograde transport of the anterograde motor, kinesin-II, and its cargo within sensory in chemosensory neurons. Therefore, the down-regulated proteins are related with the cytoskeleton arrangement, protein metabolism and cellular processes. Correspondingly, 17 differentially expressed proteins from 3day old larvae of Am fed with RJC included 6 up-regulated, and 11 down-regulated proteins. For the up-regulated proteins, pORF2 is a retrovirus reverse transcriptase with RNA-directed DNA polymerase activity. Yellow-e2 responses to fungus/G + /G2, and functions in caste determination, and is influenced by environmental factors. MDH is the key enzymes in the citric acid cycle, and possess L-malate dehydrogenase Raddeanin-A activity elevated by oxidative stress. Ornithine aminotransferase precursor possesses or nithineoxo-acid transaminase activity in metabolic process. For the down-regulated proteins, T-complex Chaperonin 5 is involved in productive folding of proteins. The methylation of the Hex110 gene in A. mellifera is regulated at the developmental stage and in a caste-dependent manner. HSP60 and Hsc70-5 are heat shock proteins responding to stress. Generally, HSPs act as molecular chaperones facillitating protein maturation in cells and activating Cyanoacetohydrazide innate and acquired immune responses. They are excellent immunomodulators against a wide variety of pathogens in protecting host. Thioredoxin peroxidase 1 is in response to oxidative stress. Elongation factor 2 is a GTPase, involved in the translocation of the peptidyl-tRNA. Fumary lace to acetase hydrolysis is responsible for the hydrolysis of fumarylacetoacetate to generate fumarate and acetoacetic acid.Putative invasion protein is a prominent inner-membrane component of the Salmonella type III secretion system apparatus, which is responsible for regulating virulence protein export in pathogenic bacteria.