Safety in silkworm egg production is critical for sericulture. Silkworm microsporidiosis is the primary reason for quarantine worldwide because of its germination transmission which can lead to devastating infectious disease. Currently, drug therapy, particularly with benzimidazoles, is used to prevent and control silkworm microsporidiosis. Albendazole is a broad-spectrum benzimidazole parasiticide. ABZ is widely applied in the livestock and poultry industry and in aquaculture because of its high effectiveness and low host toxicity. Pharmacokinetic studies of ABZ have been conducted in mice, goats, cattle, sheep, rats, dogs, and humans. The in vivo metabolism of ABZ is a pathway of ABZ to albendazole sulfoxide to albendazole sulfone to albendazole amino sulfone. ABZSO is the key active ingredient that inhibits parasites from absorbing glucose. This restricts the fumaric acid reductase system and prevents ATP production, leading to glycogen depletion, inviability and failure to reproduce. In the past decade, in research on silkworm microsporidiosis, our lab has screened reagents including ABZ, which has excellent therapeutic effects. However, the treatment and mechanism of metabolism and the bioavailability of ABZ in the silkworm are poorly understood. Few accurate methods for determining ABZ in silkworm hemolymph are available. In the past 20 years, several methods for NADH detecting benzimidazole in biological tissues or food have been developed including immunoassays, capillary electrophoresis, gas chromatography tandem spectrometry and high-performance liquid chromatography. HPLC is often coupled with a refractive index detector, ultraviolet visible detector, variable wavelength detector, fluorescence AZD8186 detector or photodiode array detector. In the early 1980s, HPLC began being used for benzimidazole detection. The US Food Safety and Inspection Service recommends HPLC to measure residual ABZ content. Comparing nonaqueous capillary electrophoresis and HPLC-UV as ABZ assays for human plasma, Prochazkova et al. reported that the sensitivity and limit of detection were similar for both techniques, but for measuring ABZO, nonaqueous capillary electrophoresis was a viable alternative to HPLC.