Future Cefoxitin sodium studies will assess the key questions of antigen localization following gemcitabine treatment and also determine how effective other chemotherapy drugs are in ��exposing�� nuclear tumor antigens to T cells. Combination chemo-immunotherapy has recently been shown to be a powerful anti-cancer strategy, provided the right combination of chemotherapy drugs and immunotherapeutic agents are used. Therefore, the data from studies like ours have the potential to inform the planning and development of future clinical protocols. For example, since TAAs like survivin and MAGE-A10 are nuclear localized in a variety of cancer types, chemotherapy that augments the cross presentation of these antigens might boost the efficacy of survivin or MAGE-A10 vaccines. Furthermore, given the crucial role that cross-presentation also plays in thymic development of tolerance to TAAs, the reduced cross-presentation of nuclear localized antigen relative to cytoplasmic and secretory antigen, may result in a peripheral T cell repertoire of nuclear-TAA reactive T cells that is less tolerant than those T cells reactive to antigen from other compartments. Thus nuclear TAAs might conceivably be better targets for cancer immunotherapy. In this regard, it may be feasible to use the increased availability of nuclear antigen after chemotherapy to ��turn tumors into their own vaccines��. This, in turn, has the potential to enhance newly emerging immunotherapies such as anti-CTLA-4/anti-PD-1/L1 immune checkpoint blockade. In summary, nuclear tumor antigen are relatively less well crosspresented to anti-tumor T cells than those from the cytoplasmic and secretory compartments, but this situation can be reversed by an apoptosis-inducing chemotherapy. In our Flunarizine 2HCl present study we have identified a novel c.956G.T mutation in EDA1 gene in an Indian family with X-linked dominant type of non-syndromic tooth agenesis affecting both primary and permanent dentition.While tooth agenesis was mainly restricted to incisors and canines in all the affected females, affected male child also showed agenesis of deciduous molar and permanent premolar which indicates that the male child is more susceptible to the pathogenic affect of the mutation.