PRMT6 localizes exclusively to the cell nucleus, exhibits automethylation, and methylates in vitro glycine and arginine-rich sequences in proteins. Methylation of polymerase b by PRMT6 was shown to increase its repair activity of damaged DNA, implicating PRMT6 as a regulator of base excision repair. PRMT6 is a negative regulator of cellular as well as viral transcriptional activation. Symmetric and asymmetric Clobenpropit dihydrobromide protein methylation by PRMT enzymes has been shown to regulate the transduction of signals to the nucleus, transcript ion regulation through nuclear receptors and RNA transport between the nucleus and cytoplasm, which suggests that modulating this signaling event may have farreaching impact. Therefore, PRMTs regulated signal transduction, transcription, RNA transport, chromatin remodeling and DNA repair through posttranslational modification of proteins during the encystment. Multifunction of PRMTs suggested PRMTs might significantly contribute to the process of encysment. e-N-trimethyllysine hydroxylase was also a specific protein in the resting cysts. It is known that TMLH is the first enzyme in the biosynthetic pathway of L-carnitine and catalyzes the formation of b-hydroxy-N-e-trimethyllysine from e-N-trimethyllysine. Carnitine is a vital compound, which plays an indispensable role in the transport of activated fatty acids across the inner mitochondrial membrane into the matrix, where b-oxidation takes place. Furthermore, carnitine is involved in the transfer of the GSK1016790A products of peroxisomal b-oxidation, including acetyl-CoA. TMLH is predominantly localized in mitochondria. The submitochondrial localization of TMLH will have implications for the substrate flow and regulation of the carnitine biosynthesis. Therefore, we inferred that TMLH had important influences on the energy metabolism of the encystment. Many experimental data have showed that calpains are a ubiquitous family of calcium-dependent cysteine proteases and play important roles in a wide range of cell stress response, cell regulatory and differentiation processes through restricting enzymolysis of various enzymes in cell and cytoskeleton protein system.