Using our model system we also examine how the two types of cheaters would interact. The major reason for this Notoginsenoside-Ft1 dynamics was the difference in the effects of inter and intra-host fitness. In order to differentially study and compare the effects of the two we defined realized fitness as the net increment in the total plasmid proportion over time t. A common problem with multi level selection is that the different levels of selection work with different net effects on the realized fitness. We took the approach of studying the nature of the relationship between fitness levels with realized fitness taking one level at a time. To analyze the effects of intra-host fitness on realized fitness, Kaempferol inter-host fitness difference was set to zero by making w=0 and the increment in the net population of plasmid over time t was monitored at different values of d. Inversely to study the realized inter-host fitness in isolation, d was set to zero and the increment in the net population of the plasmid over time t was monitored at increasing inter-host fitness. It was observed that with increasing d the realized fitness increased in a saturation curve. On the other hand, the realized fitness increased exponentially with increasing inter-host fitness difference as shown schematically in Figure 4A and B. The saturating nature of the realized fitness curve with increasing intra-host fitness was owing to the inherent limitation on growth within a host cell and a constant and limiting rate of transfer across cells. Although a plasmid with a very high copy number could replace the wild type very rapidly within a host cell clone, its spread to other cells was not equally rapid being limited by the rate of conjugal transfer which was assumed to be constant. As a result of the two different natures of the curves, with a small increment in copy number the intra-host fitness advantage dominated over the inter-host fitness but with a larger difference, the inter-host fitness increased more sharply to overcompensate for the saturating effects of intra-host fitness and resulted in a net loss to the high copy number plasmid.