However, in other disease models such as liver fibrosis, influenza infection, and pulmonary metastasis that used an anti-asialo GM1 treatment paradigm similar to one we employed, NK cell depletion resulted in dramatic phenotypes. Indeed, while anti-asialo GM1 treatment resulted in similar significant yet incomplete levels of NK cell depletion as achieved in our studies, in other in vivo models this resulted in increased influenza related mortality, liver fibrosis, and pulmonary metastases. As an alternative approach to test whether NK cells have an effect in BIPF, we adoptively transferred Roxindole hydrochloride unstimulated NK cells into recipients 12 hours before bleomycin injection. We first tracked the distribution and abundance of transferred NK cells Resiquimod during BIPF using allotypic CD45 markers to distinguish donor from recipient cells. Comparing day one to day 21 post-transfer, the percentage of donor NK cells relative to recipient NK cells decreased slightly from 2.1% to 1.0% in the spleen, indicating that,50% of the transferred cells survive for the duration of the study. Furthermore, the donor cells were recruited into the airways and lung parenchyma during BIPF, indicating that they are properly positioned to exert any possible effects. Kim et. al reported that 0.3 million transferred NKT cells protected against BIPF ; in this study we transferred 1 million NK cells per mouse and evaluated fibrosis on day 21 post-bleomycin injection. There was a significant increase in the number of BAL lymphocytes in the NK cell recipients vs. saline control, which likely reflects the added bulk of NK cells to the recruited population in the airways. Adoptively transferred NK cells did not protect against lung fibrosis in the bleomycin model; if anything, there was a trend for increased collagen deposition in the lungs in the NK cell recipient mice. Thus our data suggest that NK cells are dispensable for the development of BIPF and are unlikely to play a protective role in regulating lung fibrosis. Finally, NK cell depletion strategies have been proposed to inhibit persistent viral infection as well as to promote graft vs. tumor responses following allogeneic bone marrow cell transplantation. Our data indicate that such strategies would not contribute to the development or exacerbation of pulmonary fibrosis. Congenital stationary night blindness is a clinically and genetically heterogeneous group of non-progressive hereditary retinal disorders characterized by night blindness and decreased visual acuity.