High hepatic clearance in the circulatory system. Juxtaposition of CD14 and TLR-4 in the anterior uvea contributes to the sensitivity of iris and ciliary body to LPS. CD14 does not have a trans-membrane segment and so needs TLR-4, a trans-membrane protein, to transduce the LPS stimulation. LPS binds primarily to resident macrophages or epithelial cells lining the iris and ciliary processes. When LPS-CD14-TLR4 cluster KRX-0401 activation is inhibited by lipid raftdisrupting drug or lipid A mimetic antagonists, LPS-induced cellular cascading reaction is interrupted. We have shown in this study that GTE suppresses LPS induced elevation of CD14 and TLR-4 mRNA in the anterior uvea and the retina, suggesting that GTE treatment may alleviate ocular inflammation by suppressing formation of the LPS receptor complex. These findings are consistent with previous reports showing that EGCG is able to block the interaction between LPS and TLR-4 and downregulate TLR-4 mRNA expression in vitro. However, other receptor components, such as HSP 70/90, CXCR4, and DAF also participate in the LPS-CD14TLR4 cluster activation, probably acting as additional LPStransfer molecules. Moreover, EGCG has also been shown to suppress inflammatory responses by binding to the surface molecule 67-kDa laminin receptor, which results in reduction of expression of pro-inflammatory mediators, such as TNF-a, IL-6 and cyclooxygenase-2. Whether GTE may modulate function of these molecules remains to be investigated. Anti-inflammatory properties of GTE have been reported in other experimental disease models. Epigallocatechin-3-gallate, the major constituent of GTE, has shown to reduce expression of inflammatory biomarkers in human cell lines such as chondrocytes and corneal epithelium, and experimental models of dry eye. We have shown in the current study that LPS induced nuclear NF-kBp65 is significantly reduced after GTE treatment, probably caused by a reduced level of TLR-4-CD14 receptor complex, and leads eventually to a reduced expression of genes coding for cytokines and chemokines. To our knowledge, this is the first report to demonstrate antiinflammatory effects of GTE on acute ocular inflammation. The findings in this study support strongly that GTE is a potent therapeutic agent for treatment of acute anterior uveitis. Vascular grafts are widely used for a number of medical treatments. The mechanical and biological properties are of crucial concerns for vascular graft materials.