Ca2+ subcellular localization approaches are required to verify this hypothesis. However, Ced9expression had no effect on sodium influx which increased in dose dependent manner, similarly to that observed in K599-empty hairy roots. It has been shown that under saline stress ROS generation are induced leading to oxidative damage. In this regard, it has been suggested that anti-apoptotic genes from animals would suppress ROS generation or promote its removal in plants. However, to best of our knowledge, there are no redox studies to support this hypothesis since these conclusions were based on visual observations such as a lack of decoloration in transgenic leaves under stress conditions and chlorophyll content in salt stressed leaves. In this work, we reported redox effects of Ced-9-expression in soybean hairy roots under stress conditions. Increases in antioxidant capacity in K599-empty hairy roots could indicate a response to oxidative stress induced by hairy root death-inducing conditions; while no changes were observed between treatments in K599-CED9 hairy roots. These results demonstrated that the expression of Ced9 prevents ROS generation in hairy roots under stress conditions. On the other hand, the mammalian homologous of CED-9 may regulate metabolic efficiency in neurons through interaction with the mitochondrial F1F0 ATP synthase in the inner membrane. Likewise, Qiao et al suggested a possible contribution of Bcl-xL and Ced-9 to improved mitochondrial LEE011 membrane potential when were expressed in plants. In this regard, this work demonstrated that K599-CED-9 hairy roots had improved metabolism assessed as ATP content, particularly in severe salt conditions. Strikingly, despite of improved metabolism and tolerance to death-induced stress conditions, K599-CED9 hairy roots had a significant inhibition of its nodulation capacity. Moreover, given that cell death process is an early control of the number of nodules, we expected that the expression of Ced-9 could impact positively on the nodulation process. Taking into account that one of the main action of Ced-9 is the ionic flux control, it is possible that its expression in legume could adversely affect the ion flux signatures that occur during rhizobium perception. Likewise, It has been reported in animals that CED9 interact with proteins involved in vesicular traffic and autophagy, which in turn have participation in organogenesis events. In this regard, we have the hypothesis and also relevant unpublished data showing that CED9 expression, which have no homologues identified in plants, could affect nodule organogenesis by interacting with vesicular traffic and autophagy proteins conserved in plants. In summary, in this work we characterized the effects of Ced-9expression on soybean hairy root under different, ordered-like and necrosis-like root hair and root death-inducing conditions. In this respect, we demonstrated that part of improved tolerance given by Ced-9 expression is based on the maintenance of ionic and redox homeostasis capacity.