Members of TGFB, insulin and WNT signaling family, growth factors such as GDF9 and BMP15, and hormonal regulation of FSH, LH that are also crucial for oocyte growth and developmental competence. During in vitro maturation and fertilization, a fully grown oocyte has better competency than a growing oocyte. Oocyte developmental competence is defined as the ability of an oocyte to resume meiosis, 3,4,5-Trimethoxyphenylacetic acid cleave following fertilization, develop to the blastocyst stage, induce a pregnancy and bring offspring to term with good health. The enzyme glucose-6-phosphate dehydrogenase is minimally active in the fully grown oocytes and present at higher level in growing oocytes. The enzyme G6PD can convert the Brilliant Cresyl Blue stain from blue to colorless; thus, growing oocytes will have a colorless cytoplasm while the fully grown ones remained blue. With that BCB staining of COC could be used as a method of screening oocytes for their growth status in many species including cattle and sheep. The development of COC to competent status is taking place in follicular microenvironment in which various signal transductions and molecular interactions are taking place between the surrounding cells mediated by the follicular fluid. Follicular fluid is a product of both the transfer of blood plasma constituents that cross the ��blood-follicle barrier�� and of the secretory activity of granulosa and thecal cells. It has been recognized as a reservoir of biochemical factors useful as noninvasive predictors of oocyte quality. Follicular fluid provides an important microenvironment for oocyte maturation and contains hormones such as FSH, LH, GH, inhibin, activin, estrogens and 4-(Benzyloxy)phenol androgens, pro-apoptotic factors including TNF and Fas-ligand, proteins, peptides, amino acids, and nucleotides. Follicular fluid is at least partly responsible for subsequent embryo quality and development and has some important oocyte-related functions including maintenance of meiotic arrest, protection against proteolysis, extrusion during ovulation and as a buffer against adverse haematic influences. As follicular fluid is derived from plasma and secretions of granulosa and theca cells, it is likely that products within follicular fluid may play a role in follicle growth and oocyte developmental competence. Exosomes have been postulated to play an important role in cell�Ccell communication, either by stimulating cells directly by surface expressed ligands or by transferring molecules between them. However, the mode of exosome-cell interaction and the intracellular trafficking pathway of exosomes in their recipient cells remain unclear. Exosomes are small membrane vesicles that are released into the extracellular milieu upon the fusion of multivesicular bodies with the plasma membrane. Unlike other 
cell-secreted vesicles, exosomes are more homogenous with a size range from 40-100 nm in diameter. Exosomes contain a characteristic composition of proteins, and express cell recognition molecules on their surface that facilitates their selective targeting of and uptake by recipient cells. They are natural carriers of variety of coding and non-coding RNA, including microRNAs, which can be transported over large distances through blood to recipient cells and induce de novo transcriptional and translational changes in the target cells. These findings support the idea that exosomes might constitute an exquisite mechanism for local and systemic intercellular transfer not only of proteins but also of genetic information in the form of RNA. Currently, the role of exosomes, present in bovine follicular fluid, in transporting extra-cellular miRNAs within follicular environment and their contribution to follicular growth and oocyte maturation are unknown. During the dynamic phase of follicular development and oocyte maturation, miRNAs play an important role by coordinating.