The validity of this clinical approach is well established in patients with type 1 diabetes but not in those with type 2 diabetes. Furthermore, non-diabetic renal diseases such as minimal change disease or idiopathic membranous nephropathy, either isolated or superimposed on an underlying DN, have been reported. The prevalence of biopsy-proven NDRD in patients with diabetes varies from among reports. These differences may be due to selection criteria, biopsy threshold, or the populations being studied. Treatments for DN and NDRD are quite different. Many NDRD lesions can be treated with immunosuppressants other than the standard angiotensin-converting enzyme inhibitors or angiotensin receptor blockers. Thus, it is important to distinguish NDRD from DN early. A kidney biopsy is necessary to confirm the diagnosis, but is invasive. Nephrologists are sometimes reluctant to perform a renal biopsy on patients with DM because of the potential risks of the procedure such as hematuria, perirenal hematoma, arterial embolization, and even the necessity for a nephrectomy. Moreover, there exist some contraindications for renal biopsy such as the solitary kidney and cortical atrophy. Additionally, many primary hospitals are at present unable to perform the renal biopsy. Therefore, nephrologists must provide a suspected diagnosis using the clinical and laboratory data available before a biopsy is performed.Each of these steps is precisely regulated by the reciprocal communication between glial cells and neurons. The molecular mechanisms that mediate the axonal-glial interaction and myelin formation in the CNS remain elusive. Which can be difficult to probe by endpoint assessment in pharmacology. IPA is a non-polar solvent that is used to dissolve LIG in the mobile phase for HPLC to separate LIG from other RASextracted compounds. LIG dissolved in IPA can be homogeneously distributed to cell media. However, our study shows that IPA alone could slightly alter the single cell migration pattern but not the wound-like gap closure process involving cellcell interactions. Hence, the presence of IPA may directly affect cell behavior by hindering the cell migration capacity but this effect could be shielded by cell-cell interactions through signaling crosstalk. There is another possibility: the evaporation of IPA during the incubation period could also make the IPA’s effect inconsistent. Since the wound-like gap closure process takes much longer than the single cell assay, the IPA’s effect on T98G cells could be gradually diminished and down-played during the time course of the wound-like gap closure process.