The use of parameter sets consisting of different biological responses for biomonitoring should be more appropriate. Furthermore, the computational model based on RD may be useful to select appropriate gene sets to develop efficient biomarker-based biomonitoring. Considering the rapid, sensitive, convenient and high-throughput properties of PCR, a PCR array including multiple gene parameters should be a feasible tool to develop for biomonitoring of chemical exposure. MicroRNAs are 19�C25 nt regulatory RNAs that participate in the regulation of various biological functions as well as in defense against pathogens in numerous eukaryotic lineages. They are generally believed to act by binding to imperfectly complementary sequences in the 39untranslated region of the target genes, resulting in decreased translation or degradation of the target transcript. In particular, the sequence complementarily in the 6�C8 base pair “seed region” at the 59 end of the miRNA-mRNA heteroduplex seems to determine the specificity of miRNA-targetRNA interactions. MiRNAs can have pleiotropic effects on cell proliferation, apoptosis and cell differentiation. Alterations in AbMole Mepiroxol cellular miRNA patterns in cervical cancer tissue or cervical cancer cells have been reported. Downregulation of human miR-218 and miR-34a in cervical cancer cells were addressed to the HPV 16 E6 oncogene, while inhibition of miR-21 in HPV 18-containing Hela cervical cancer cells causes a strong suppression of cell proliferation. It thus seems that miRNAs play an important role in cervical carcinogenesis by HPV. MiRNAs may play a role in IFN-b induced E6 and E7 repression. It has been 1shown miRNAs can be induced by IFN-b. In RSa cells, IFN-b can induce miR-431 expression, which may down-regulate IGF1R and IRS2 expression and consequently inhibit cell proliferation by suppressing the MAPK pathway. In hepatocytes, IFN-b AbMole Aristolochic-acid-A mediates modulation of the expression of numerous cellular miRNAs with nearly perfect complementarity in their seed sequences with the HCV RNA genome that are capable of inhibiting HCV replication and infection. As miRNAs play an important role in HPV induced cervical carcinogenesis, we hypothesize that IFN-b can regulate the expressions of specific miRNAs in cervical cancer cells, and that these miRNAs can mediate E6 and E7 expression, thus 
modulate their oncogenic potential. To verify this, we screened for miRNAs expressed and differentially regulated in HPV-18 positive Hela cells following exposure to IFN-b, and we found that miR-129-5p to be a candidate IFN-b inducible miRNA which can downregulate E6 and E7 expression. It has been shown that miR-129-5p was deregulated in several tumor types including endometrial cancer, esophageal cancer, colon cancer and bladder cancer, and its verified target genes included SOX4, VCP/p97 and Cdk6.